Selected article for: "real time and RNA Extraction kit"
Author: Marriott, Andrew S.; Vasieva, Olga; Fang, Yongxiang; Copeland, Nikki A.; McLennan, Alexander G.; Jones, Nigel J.
Title: NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap(4)A) and Down-Regulates Immune Response and Cancer Promotion Genes
  • Document date: 2016_5_4
    • ID: 0ozzbp85_13
    Snippet: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro cDNA synthesis kit, both according to the manufacturer's instructions. The cDNA was then quantitated by PCR using Maxima SYBR Green master mix (Thermo) and a StepOnePlus™ Real Time PCR system (Applied Biosystems). Primers were obtained from Sigma and are listed in S1 Table. The 2 -ΔΔCt m.....
    Document: RT-PCR analysis of selected genes RNA extraction was performed using a Qiagen RNeasy mini kit with QIAshredder and cDNA was synthesized using a Bioline Tetro cDNA synthesis kit, both according to the manufacturer's instructions. The cDNA was then quantitated by PCR using Maxima SYBR Green master mix (Thermo) and a StepOnePlus™ Real Time PCR system (Applied Biosystems). Primers were obtained from Sigma and are listed in S1 Table. The 2 -ΔΔCt method was used to determine relative transcript levels using the housekeeping GAPDH gene to normalize the data [45] .

    Search related documents:
    Co phrase search for related documents
    • Applied Biosystems real Time pcr system and GAPDH gene: 1, 2
    • Applied Biosystems real Time pcr system and master mix: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • Applied Biosystems real Time pcr system and mini kit: 1, 2
    • Applied Biosystems real Time pcr system and pcr system: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • Applied Biosystems real Time pcr system and real Time pcr system: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • Applied Biosystems real Time pcr system and relative transcript: 1
    • Applied Biosystems real Time pcr system and RNA extraction: 1, 2, 3
    • cdna synthesis and GAPDH gene: 1, 2, 3, 4
    • cdna synthesis and housekeeping GAPDH gene: 1, 2
    • cdna synthesis and master mix: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • cdna synthesis and mini kit: 1, 2, 3, 4, 5
    • cdna synthesis and PCR quantitate: 1
    • cdna synthesis and pcr system: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • cdna synthesis and real Time pcr system: 1, 2, 3, 4, 5, 6
    • cdna synthesis and RNA extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • cdna synthesis kit and GAPDH gene: 1, 2
    • cdna synthesis kit and housekeeping GAPDH gene: 1
    • cdna synthesis kit and master mix: 1, 2, 3, 4, 5, 6, 7
    • cdna synthesis kit and mini kit: 1, 2