Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China

Qin, Si-Yuan; Cong, Wei; Liu, Ye; Li, Nan; Wang, Ze-Dong; Zhang, Fu-Kai; Huang, Si-Yang; Zhu, Xing-Quan; Liu, Quan

The present study revealed that T. gondii prevalence was higher in bats in southern China (Guangdong, and Jiangxi) than in northern China (Jilin, and Liaoning). The possible reason is that warm and humid environment in II/III II II II II II II II II II II Reference, Type II, ToxoDB #1 CTG Cat United States II/III III III III III III III III III III I III III III III III I I I u-1 * I Reference, ToxoDB #19 TgWtdSc40 WTD USA u-1 II II II II II II II I II I Type 12, ToxoDB #5 TgCatBr64 Cat Brazil I I u-1 III III III u-1 I III III I Reference, ToxoDB #111 TgRsCr1 Toucan Costa Rica u-1 I II III I III u-2 I I III I Reference, ToxoDB #52 TgBatJL1-TgBatJL2 MC Liver Jilin, China u-1 II II III III II II III II II I ToxoDB #9 TgBatJL3 ML Liver Jilin, China I I I I I I I I I I I ToxoDB #10 TgBatJL4 ML Liver Jilin, China I I I I nd I I I I nd nd =nd TgBatJX5-TgBatJX7 RF Liver Jiangxi, China u-1 II II III III II II III II II I ToxoDB #9 TgBatGD8 CS Liver Guangdong, China I I I I I I I nd I I nd nd TgBatGD9, TgBatGD10 CS Liver Guangdong, China I I I I I I I I I I I ToxoDB #10 TgBatGD11 CS Liver Guangdong, China nd I nd I I I I I I nd southern China is more suitable for survival of T. gondii oocysts [1] . The 38 T. gondii-positive bat samples were directly genotyped, and only 8 positive DNA samples were completely genotyped, possibly due to low DNA concentration. Of which, 3 samples (2 from C. sphinx, and 1 from M. leucogaster) represented ToxoDB Genotype #10, and 5 samples (2 from M. leucogaster, 2 from M. chinensis, and 1 from R. ferrumequinum) belonged to ToxoDB Genotype #9 (Table 1) .