Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches

Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian

DCs can uptake antigens from the gut lumen by extending dendrites into epithelium, then process and present to lymphocytes, which effectively antigen produces an immune response [4, 14, 15] . So in next step, we investigated the potential of DCs to prime lymphocytes responses after its interactions with lymphocytes. As the result showed, DCs in porcine PPs from oral inactivated PEDV pigs for two months had a significantly increased ability to promote lymphocytes proliferation (Fig. 2a, b) , while that of DCs from conventional healthy pigs had no significant changes (Fig. 2b) . Fig. 2 DCs can promoted the proliferation of allogeneic lymphocytes. a DCs in PPs from conventional healthy pigs and oral inactivated PEDV for two months co-cultured with allogeneic lymphocytes at a ratio of 1:10.lymphocytes proliferation was analyzed by CCK8. b DCs from intestinal mucosal PPs from conventional healthy pigs and oral inactivated PEDV for two months co-cultured with CFSE-labeled allogeneic lymphocytes at a ratio of 1:10.lymphocytes proliferation was analyzed by CFSE dilution using FACS. Percentages refer to proportion of lymphocytes that proliferated within 5 days. Data are represented as mean ± S.D. One representative of three similar independent experiments is shown