Author: Sugiarto, Sarah; Spiri, Andrea M.; Riond, Barbara; Novacco, Marilisa; Oestmann, Angelina; de Miranda, Luisa H. Monteiro; Meli, Marina L.; Boretti, Felicitas S.; Hofmann-Lehmann, Regina; Willi, Barbara
Title: Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis Document date: 2016_8_5
ID: 1uw8xcxw_24
Snippet: Flow cytometric analysis was performed on day 0 and on days 3, 7, 14, 21, 28, 42, 56, 70 and 98 pi. Three different staining combinations of primary antibodies were used: (1) Fluorescein isothiocyanate (FITC)-conjugated mouse anti-feline CD5 antibody (f43, Southern Biotech, Allschwil, Switzerland); (2) an R-phycoerythrin (RPE)conjugated mouse anti-feline CD4 antibody (Vpg34, AbD Serotec, Puchheim, Germany) and a FITC-conjugated mouse anti-feline .....
Document: Flow cytometric analysis was performed on day 0 and on days 3, 7, 14, 21, 28, 42, 56, 70 and 98 pi. Three different staining combinations of primary antibodies were used: (1) Fluorescein isothiocyanate (FITC)-conjugated mouse anti-feline CD5 antibody (f43, Southern Biotech, Allschwil, Switzerland); (2) an R-phycoerythrin (RPE)conjugated mouse anti-feline CD4 antibody (Vpg34, AbD Serotec, Puchheim, Germany) and a FITC-conjugated mouse anti-feline CD25 antibody; and (3) a FITC-conjugated mouse anti-feline CD8 antibody (fCD8, Southern Biotech) and a RPE-conjugated rat anti-mouse CD45R/ B220 antibody (RA3-6B2, AbD Serotec). CD5 is a marker for feline T lymphocytes [34] , and CD4 + -and CD8 +positive T lymphocytes represent helper and cytotoxic T lymphocytes, respectively [35] . CD4 + CD25 + T lymphocytes represent activated CD4 + T lymphocytes, and CD45R/B220 antibody was used to identify B lymphocytes [36, 37] .
Search related documents:
Co phrase search for related documents- cytometric analysis and fluorescein isothiocyanate: 1, 2, 3, 4
- FITC conjugate and fluorescein isothiocyanate: 1
- FITC conjugate and primary antibody: 1
- flow cytometric analysis and fluorescein isothiocyanate: 1, 2, 3, 4
- fluorescein isothiocyanate and primary antibody: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date