Author: Sugiarto, Sarah; Spiri, Andrea M.; Riond, Barbara; Novacco, Marilisa; Oestmann, Angelina; de Miranda, Luisa H. Monteiro; Meli, Marina L.; Boretti, Felicitas S.; Hofmann-Lehmann, Regina; Willi, Barbara
Title: Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis Document date: 2016_8_5
ID: 1uw8xcxw_25
Snippet: Prior to the start of the experiment, the antibodies were titrated for optimal dilution as follows: CD4 and CD8, 1:50 dilution; CD25 and CD5, 1:100 dilution; and CD45R/B220, undiluted. A 50 µL aliquot of EDTA anticoagulated blood was incubated with 5 µL of the aforementioned antibodies. To rule out non-specific binding, isotype control antibodies (RPE-conjugated Mouse IgG1 and FITC-conjugated Mouse IgG1, AbD Serotec) and unstained blood samples.....
Document: Prior to the start of the experiment, the antibodies were titrated for optimal dilution as follows: CD4 and CD8, 1:50 dilution; CD25 and CD5, 1:100 dilution; and CD45R/B220, undiluted. A 50 µL aliquot of EDTA anticoagulated blood was incubated with 5 µL of the aforementioned antibodies. To rule out non-specific binding, isotype control antibodies (RPE-conjugated Mouse IgG1 and FITC-conjugated Mouse IgG1, AbD Serotec) and unstained blood samples from each cat were used as negative controls. Blood samples were stained according to published protocols [27, 28] .
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