Author: Sugiarto, Sarah; Spiri, Andrea M.; Riond, Barbara; Novacco, Marilisa; Oestmann, Angelina; de Miranda, Luisa H. Monteiro; Meli, Marina L.; Boretti, Felicitas S.; Hofmann-Lehmann, Regina; Willi, Barbara
Title: Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis Document date: 2016_8_5
ID: 1uw8xcxw_60
Snippet: Several measures were undertaken to ensure that the plasma used for passive immunization did not contain viable Mhf organisms that could transmit infection. The donor cats were tested weekly for at least eight consecutive weeks before the plasma was collected. All these samples and the plasma pool itself were tested in triplicate with a highly sensitive Mhf-specific qPCR assay [12] and revealed PCR-negative results. Furthermore, an aliquot of the.....
Document: Several measures were undertaken to ensure that the plasma used for passive immunization did not contain viable Mhf organisms that could transmit infection. The donor cats were tested weekly for at least eight consecutive weeks before the plasma was collected. All these samples and the plasma pool itself were tested in triplicate with a highly sensitive Mhf-specific qPCR assay [12] and revealed PCR-negative results. Furthermore, an aliquot of the plasma pool used for passive immunization was transfused into an adult SPF, the cat was followed for 23 weeks after transfusion and stayed PCR-negative.
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