Selected article for: "antibody light and heavy chain antibody light"
Author: Yong, Kylie Su Mei; Ng, Justin Han Jia; Her, Zhisheng; Hey, Ying Ying; Tan, Sue Yee; Tan, Wilson Wei Sheng; Irac, Sergio Erdal; Liu, Min; Chan, Xue Ying; Gunawan, Merry; Foo, Randy Jee Hiang; Low, Dolyce Hong Wen; Mendenhall, Ian Hewitt; Chionh, Yok Teng; Dutertre, Charles-Antoine; Chen, Qingfeng; Wang, Lin-Fa
Title: Bat-mouse bone marrow chimera: a novel animal model for dissecting the uniqueness of the bat immune system
  • Document date: 2018_3_16
    • ID: 01f36rld_25
    Snippet: ELISA. Sera were collected before and after challenging with NP-KLH by cheek bleed. Bat immunoglobulin (Ig) content specific to NP-KLH antigens was assessed by ELISA. Briefly, microtiter plates (Thermo Fisher Scientific, USA) were coated with NP31-BSA (Biosearch Technologies, USA) at 10 µg/mL. Bat specific antibodies were detected with bat IgG heavy and light chain antibody (A140-118B; Bethyl Laboratories, USA), donkey anti-goat IgG-HRP (SC-2056.....
    Document: ELISA. Sera were collected before and after challenging with NP-KLH by cheek bleed. Bat immunoglobulin (Ig) content specific to NP-KLH antigens was assessed by ELISA. Briefly, microtiter plates (Thermo Fisher Scientific, USA) were coated with NP31-BSA (Biosearch Technologies, USA) at 10 µg/mL. Bat specific antibodies were detected with bat IgG heavy and light chain antibody (A140-118B; Bethyl Laboratories, USA), donkey anti-goat IgG-HRP (SC-2056; Santa Cruz Biotechnology, USA) and 3,3′,5,5′-tetramethylbenzidine (TMB) (Thermo Fisher Scientific, USA). Optical density (OD) was read at 450 nm with a plate reader (Tecan, USA).

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