MyD88-dependent influx of monocytes and neutrophils impairs lymph node B cell responses to chikungunya virus infection via Irf5, Nos2 and Nox2

McCarthy, Mary K.; Reynoso, Glennys V.; Winkler, Emma S.; Mack, Matthias; Diamond, Michael S.; Hickman, Heather D.; Morrison, Thomas E.

Mice were anesthetized with isoflurane and inoculated with 10 3 PFU of CHIKV subcutaneously in the left rear footpad in a volume of 10 μL of PBS/1% FBS. Mice were sacrificed after exposure to isoflurane vapors followed by thoracotomy and perfusion with PBS at the indicated time points. In some experiments, mice were treated with 300-500 μg of IgG2b isotype control Ab (LTF-2), anti-Gr-1 mAb (RB6-8C5), IgG2a isotype control Ab (2A3), or anti-Ly-6G mAb (1A8) (all from BioXCell) i.p. the day prior to infection. For IL-1R blockade, mice were treated with 200 μg Armenian hamster IgG control Ab or anti-IL-1R Ab (JAMA-147) (both from BioXCell) at the time of infection.