Author: Medigeshi, Guruprasad R.; Kumar, Rinki; Dhamija, Ekta; Agrawal, Tanvi; Kar, Meenakshi
Title: N-Desmethylclozapine, Fluoxetine, and Salmeterol Inhibit Postentry Stages of the Dengue Virus Life Cycle Document date: 2016_10_21
ID: 0nm7wgf5_2
Snippet: Kang. Rotavirus stock preparation and infection of Caco-2 cells (MOI of 0.5 27 pfu/cell) was performed as described previously except that serum free medium 28 was used at all stages (1). For determination of viral titers, infected culture 29 supernatants were activated by trypsin as described above, added on MA-104 30 cells were determined and continued incubation for one hour at 37° C. Virus 31 inoculum was removed and overlay medium (MEM with.....
Document: Kang. Rotavirus stock preparation and infection of Caco-2 cells (MOI of 0.5 27 pfu/cell) was performed as described previously except that serum free medium 28 was used at all stages (1). For determination of viral titers, infected culture 29 supernatants were activated by trypsin as described above, added on MA-104 30 cells were determined and continued incubation for one hour at 37° C. Virus 31 inoculum was removed and overlay medium (MEM with 0.8% tracaganth and 0.5 32 µg/ml trypsin) was added. 3 days post-infection, overlay medium was removed 33 and cells were fixed and stained as per the protocol followed for DENV. 34
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