Author: O’Connell, Grant C.; Treadway, Madison B.; Petrone, Ashley B.; Tennant, Connie S.; Lucke-Wold, Noelle; Chantler, Paul D.; Barr, Taura L.
Title: Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption Document date: 2017_4_26
ID: 1ey6ie95_19
Snippet: Microarray. RNA was amplified and biotinylated using the TotalPrep RNA amplification kit (Applied Biosystems, Grand Island, NY). Samples were hybridized to HumanRef-8 expression bead chips (Illumina, San Diego, CA) and scanned using the Illumina BeadStation. Raw probe intensities were background subtracted, quantile normalized, and then summarized at the gene level (total gene expression) using Illumina GenomeStudio. Sample labeling, hybridizatio.....
Document: Microarray. RNA was amplified and biotinylated using the TotalPrep RNA amplification kit (Applied Biosystems, Grand Island, NY). Samples were hybridized to HumanRef-8 expression bead chips (Illumina, San Diego, CA) and scanned using the Illumina BeadStation. Raw probe intensities were background subtracted, quantile normalized, and then summarized at the gene level (total gene expression) using Illumina GenomeStudio. Sample labeling, hybridization, and scanning were performed per standard Illumina protocols. mRNA expression values are presented as normalized mean probe intensity. Raw data are available via NCBI Gene Expression Omnibus (GEO) accession GSE16561.
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