Author: Hedegaard, Chris J.; Strube, Mikael L.; Hansen, Marie B.; Lindved, Bodil K.; Lihme, Allan; Boye, Mette; Heegaard, Peter M. H.
Title: Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs Document date: 2016_1_29
ID: 1tcpaigw_28
Snippet: To follow shedding of the E. coli F4+O149 infection throughout the experiment, a qPCR specific for the F4 gene was performed on faecal samples as described by Ståhl et. al. [21] . Next generation 16S rDNA sequencing DNA was purified from the ileum samples and the V1-V2 regions of 16S rRNA gene was amplified by PCR and sequenced as described by Strube et al. [22] . Briefly, the 16S rRNA gene amplicons were submitted to the DTU Multi-Assay Core (h.....
Document: To follow shedding of the E. coli F4+O149 infection throughout the experiment, a qPCR specific for the F4 gene was performed on faecal samples as described by Ståhl et. al. [21] . Next generation 16S rDNA sequencing DNA was purified from the ileum samples and the V1-V2 regions of 16S rRNA gene was amplified by PCR and sequenced as described by Strube et al. [22] . Briefly, the 16S rRNA gene amplicons were submitted to the DTU Multi-Assay Core (https://dmac.cbs.dtu.dk/) and sequenced on the MiSeq platform (Illumina Inc. San Diego, USA). The resulting sequences were then merged, quality filtered and mapped against the RDP-II SSU database [23] using the BION-meta software (Danish Genome Institute, Aarhus, Denmark). The resulting read counts were then analysed on the family level. Data were uploaded to Genbank database: http://www. ncbi.nlm.nih.gov/sra/SRP066524.
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