Author: Guanmin Jiang; Xiaoshuai Ren; Yan Liu; Hongtao Chen; Wei Liu; Zhaowang Guo; Yaqin Zhang; Chaoqun Chen; Jianhui Zhou; Qiang Xiao; Hong Shan
Title: Application and optimization of RT-PCR in diagnosis of SARS-CoV-2 infection Document date: 2020_2_27
ID: 65plab8b_8
Snippet: For this retrospective, single center study, we recruited 584 patients from Jan 17 to Feb 11, 2020, at The Fifth Affiliated Hospital of Sun Yat-sen University in Zhuhai, China, which is a designated infectious hospital. During this period, RT-PCR and chest CT was performed for consecutive patients including the local residents of Wuhan, outside of Wuhan did have a recent travel to Wuhan or contact with people with fever or respiratory symptoms fr.....
Document: For this retrospective, single center study, we recruited 584 patients from Jan 17 to Feb 11, 2020, at The Fifth Affiliated Hospital of Sun Yat-sen University in Zhuhai, China, which is a designated infectious hospital. During this period, RT-PCR and chest CT was performed for consecutive patients including the local residents of Wuhan, outside of Wuhan did have a recent travel to Wuhan or contact with people with fever or respiratory symptoms from Wuhan, or had fever or acute respiratory symptoms of unknown cause. Of the 16 patients recruited as of Feb 11, had a suspected diagnosis and were therefore excluded in this study. 87 patients, who were diagnosed as having COVID-19 and 481 patients exclusion COVID-19 according to WHO interim guidance, were enrolled in this study. The performances of the first RT-PCR detection in pharyngeal swabs and chest CT were evaluated by sensitivity, specificity, youden's index et al. Then the performances of combination of the second RT-PCR, or chest CT were also calculated. Agreement between the two method was analyzed using
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