Author: Hedegaard, Chris J.; Strube, Mikael L.; Hansen, Marie B.; Lindved, Bodil K.; Lihme, Allan; Boye, Mette; Heegaard, Peter M. H.
Title: Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs Document date: 2016_1_29
ID: 1tcpaigw_18
Snippet: One day before the assay was carried out, 100 μl (15,000 cells) neonatal porcine jejunum derived IPEC-J2 cells (ACC-701; DSMZ, Braunschweig, Germany) were seeded in each well of a 96-well plate (NUNC) and were allowed to attach for 16 hrs. On the same plate, control wells received 100 μl of the cell culture medium (Advanced DMEM/F12 supplemented with 5% heat-inactivated foetal bovine serum, 2 mM GlutaMAX, 100 units/ml penicillin, 100 μg/ ml st.....
Document: One day before the assay was carried out, 100 μl (15,000 cells) neonatal porcine jejunum derived IPEC-J2 cells (ACC-701; DSMZ, Braunschweig, Germany) were seeded in each well of a 96-well plate (NUNC) and were allowed to attach for 16 hrs. On the same plate, control wells received 100 μl of the cell culture medium (Advanced DMEM/F12 supplemented with 5% heat-inactivated foetal bovine serum, 2 mM GlutaMAX, 100 units/ml penicillin, 100 μg/ ml streptomycin, and 2 μg/ml Fungizone; all purchased at Life Technologies) without IPEC-J2 cells.
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