Author: Hedegaard, Chris J.; Strube, Mikael L.; Hansen, Marie B.; Lindved, Bodil K.; Lihme, Allan; Boye, Mette; Heegaard, Peter M. H.
Title: Natural Pig Plasma Immunoglobulins Have Anti-Bacterial Effects: Potential for Use as Feed Supplement for Treatment of Intestinal Infections in Pigs Document date: 2016_1_29
ID: 1tcpaigw_8
Snippet: Samples for SDS PAGE were mixed with 4X LDS Sample buffer and 10X reducing agent (Life Technologies, Taastrup, Denmark). For SDS PAGE Novex Bis-Tris 12%, 1 mm polyacrylamide gels were used in the NuPAGE buffer system according to the manufacturer's instructions (Life Technologies), loading 20 μl sample in each well. After electrophoresis, gels were stained with Bio-Safe™ Coomassie G-250 stain (Bio-Rad, Copenhagen, Denmark)......
Document: Samples for SDS PAGE were mixed with 4X LDS Sample buffer and 10X reducing agent (Life Technologies, Taastrup, Denmark). For SDS PAGE Novex Bis-Tris 12%, 1 mm polyacrylamide gels were used in the NuPAGE buffer system according to the manufacturer's instructions (Life Technologies), loading 20 μl sample in each well. After electrophoresis, gels were stained with Bio-Safe™ Coomassie G-250 stain (Bio-Rad, Copenhagen, Denmark).
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