miR-142-5p Disrupts Neuronal Morphogenesis Underlying Porcine Hemagglutinating Encephalomyelitis Virus Infection by Targeting Ulk1

Li, Zi; Lan, Yungang; Zhao, Kui; Lv, Xiaoling; Ding, Ning; Lu, Huijun; Zhang, Jing; Yue, Huiqing; Shi, Junchao; Song, Deguang; Gao, Feng; He, Wenqi

Briefly, 3-week-old BALB/c mice (male) were intranasally inoculated with 0.1 mL PHEV (10 4.45 50% tissue culture infectious doses [TCID 50 ]/0.1 mL, GenBank: AY078417.1) or equal amounts of phosphate-buffered saline (PBS, 1 M, pH 7.4). At 24 h after PHEV infection, the inoculated mice were anesthetized with 3.5% chloral hydrate (1.0 mL/100 g; Sigma, USA), and 10 nmoL miR-142-5p antagomirs or negative control antagomirs were then positioned in the cerebral cortex with a stereotaxic apparatus (David Kopf Instruments, Tujunga, CA, U.S.A) using previously described methods (Kirby et al., 2012; Wu et al., 2012) . The antagomirs used here, are a class of chemically engineered and modified oligonucleotides that prevent other molecules from binding to a desired site on an mRNA molecule, and purchased from RiboBio in China. Mice were sacrificed at various timepoints, and the cerebral cortices of PHEV-or control-infected mice were dissected for the following study.