Author: Li, Zhao; Liu, Yong; Wei, Qingquan; Liu, Yuanjie; Liu, Wenwen; Zhang, Xuelian; Yu, Yude
Title: Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids Document date: 2016_4_13
ID: 0tmd9knh_8
Snippet: To covalently couple methoxy-PEG-silane (2-[methoxy(polyethyleneoxy)propyl]-trimethoxysilane; J&K Scientific, Beijing, China) to the PWA chip surface, the bare chips were treated in an oxygen plasma for 30 s to clean the surfaces (Mercator Control Systems, Inc., Santa Ana, CA, USA). Then, they were placed in 1 g of methoxy-PEG-silane dissolved in 100 mL of anhydrous toluene with 1% triethylamine as a catalyst overnight at room temperature [68, 69.....
Document: To covalently couple methoxy-PEG-silane (2-[methoxy(polyethyleneoxy)propyl]-trimethoxysilane; J&K Scientific, Beijing, China) to the PWA chip surface, the bare chips were treated in an oxygen plasma for 30 s to clean the surfaces (Mercator Control Systems, Inc., Santa Ana, CA, USA). Then, they were placed in 1 g of methoxy-PEG-silane dissolved in 100 mL of anhydrous toluene with 1% triethylamine as a catalyst overnight at room temperature [68, 69] . Before the application of the PWA chip, the physically adsorbed methoxy-PEG-silane moieties were removed by rinsing the chip in ethanol and DI water, sequentially. Finally, the PWA chip was dried with nitrogen and used immediately.
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