Author: Steelman, Andrew J; Li, Jianrong
Title: Poly(I:C) promotes TNFa/TNFR1-dependent oligodendrocyte death in mixed glial cultures Document date: 2011_8_3
ID: 16032h3d_24
Snippet: Poly(I:C) is not directly toxic to pre-oligodendrocytes As oligodendrocytes, astrocytes and microglia reportedly express TLR3 [7, 15] , a prominent receptor for poly(I:C), it was possible that preOLs in mixed glial cultures were dying in a cell autonomous fashion in response to poly(I: C). Consistent with previous reports, western blot analysis revealed that all glial cells expressed TLR3 in culture ( Figure 3A) . To examine the direct effects of.....
Document: Poly(I:C) is not directly toxic to pre-oligodendrocytes As oligodendrocytes, astrocytes and microglia reportedly express TLR3 [7, 15] , a prominent receptor for poly(I:C), it was possible that preOLs in mixed glial cultures were dying in a cell autonomous fashion in response to poly(I: C). Consistent with previous reports, western blot analysis revealed that all glial cells expressed TLR3 in culture ( Figure 3A) . To examine the direct effects of poly (I:C) on preOLs, purified preOL cultures were stimulated with increasing concentrations of poly(I:C) and cell viability was determined. In contrast to poly(I:C)-induced preOL death in mixed glial cultures, we found that poly (I:C) was not toxic to pure preOLs ( Figure 3B-C) . Instead, poly(I:C) treatment appeared to result in increased viability, but this effect was not statistically significant.
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