Author: Jiang, Yuting; Zhao, Guangyu; Song, Nianping; Li, Pei; Chen, Yuehong; Guo, Yan; Li, Junfeng; Du, Lanying; Jiang, Shibo; Guo, Renfeng; Sun, Shihui; Zhou, Yusen
Title: Blockade of the C5a–C5aR axis alleviates lung damage in hDPP4-transgenic mice infected with MERS-CoV Document date: 2018_4_24
ID: 0kihygau_18
Snippet: Sections of paraffin-embedded spleen (4 μm thickness) were prepared to assess splenic cell apoptosis using a TdT In Situ Apoptosis Detection Kit-DAB TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling)based Apoptosis Detection Assay according to the manufacturer's protocols (Roche, Germany). Briefly, after dewaxation and treatment with proteinase K, sections were labeled with labeling solution. After signal conversion using a Cov.....
Document: Sections of paraffin-embedded spleen (4 μm thickness) were prepared to assess splenic cell apoptosis using a TdT In Situ Apoptosis Detection Kit-DAB TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling)based Apoptosis Detection Assay according to the manufacturer's protocols (Roche, Germany). Briefly, after dewaxation and treatment with proteinase K, sections were labeled with labeling solution. After signal conversion using a Coverter-POD, the signal was detected and analyzed by light microscopy. The number of apoptotic cells in 10 random high-power fields (hpf) (×400 magnification) were calculated, and the results were expressed as TUNEL-positive cells/hpf.
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