Selected article for: "confocal microscopy and wild type peptide"
Author: de Melo, Ivan S.; Jimenez-Nuñez, Maria D.; Iglesias, Concepción; Campos-Caro, Antonio; Moreno-Sanchez, David; Ruiz, Felix A.; Bolívar, Jorge
Title: NOA36 Protein Contains a Highly Conserved Nucleolar Localization Signal Capable of Directing Functional Proteins to the Nucleolus, in Mammalian Cells
  • Document date: 2013_3_13
    • ID: 0jx6mwiw_32
    Snippet: which is a frequent feature in the nucleolar localization signals identified in other proteins [46, 47] . These constructs, together with the eGFP empty vector were transiently transfected in HeLa cells, and the eGFP localization was visualized by fluorescence microscopy (Fig. 4C) . Western blots analysis of the different mutants proteins with an anti-eGFP antibody confirmed that the full length proteins were expressed (Fig. 4B) . These transfect.....
    Document: which is a frequent feature in the nucleolar localization signals identified in other proteins [46, 47] . These constructs, together with the eGFP empty vector were transiently transfected in HeLa cells, and the eGFP localization was visualized by fluorescence microscopy (Fig. 4C) . Western blots analysis of the different mutants proteins with an anti-eGFP antibody confirmed that the full length proteins were expressed (Fig. 4B) . These transfectants were also analyzed by confocal microscopy in order to quantify the eGFP intensity in the nucleolus and in the nucleus (excluding the nucleolus) (Fig. 4D ). This quantification revealed that the wild-type peptide (1-33) showed a nucleolus/nucleus ratio of 9.8, versus a ratio of 1.1 in the eGFP empty vector.

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