Selected article for: "cdna complementary dna and dna virus"
Author: Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing
Title: Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway
  • Document date: 2015_8_18
    • ID: 0qt7eth0_21
    Snippet: Total RNA was extracted from the transfected cells using TriQuick reagent (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) according to the manufacturer's instructions and then reverse-transcribed into complementary DNA (cDNA) using murine leukemia virus reverse transcriptase (GBI Labs/Golden Bridge International, Inc., Mukilteo, WA, USA) with oligo dT random hexamers (HaiGene Technology, Harbin, China). The cDNA was then subject.....
    Document: Total RNA was extracted from the transfected cells using TriQuick reagent (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) according to the manufacturer's instructions and then reverse-transcribed into complementary DNA (cDNA) using murine leukemia virus reverse transcriptase (GBI Labs/Golden Bridge International, Inc., Mukilteo, WA, USA) with oligo dT random hexamers (HaiGene Technology, Harbin, China). The cDNA was then subjected to real-time PCR with specific primer pairs targeting IFN-β (F: 5′-GCTAAC AAGTGCATCCTCCAAA-3′ and R: 5′-CCAGGAGC TTCTGACATGCCA-3′) and β-actin (F: 5′-GGCTCAG AGCAAGAGAGGTATCC-3′, and R: 5′-GGTCTCAAA CATGATCTGAGTCATCT-3′. β-actin mRNA was used as an endogenous control.

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