Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

Klaile, Esther; Klassert, Tilman E; Scheffrahn, Inka; Müller, Mario M; Heinrich, Annina; Heyl, Kerstin A; Dienemann, Hendrik; Grünewald, Christiane; Bals, Robert; Singer, Bernhard B; Slevogt, Hortense

BALF samples were obtained from 181 individuals in whom a bronchoscopy was performed for different diagnostic or therapeutic purposes. All individuals underwent bronchoscopy following standard diagnostic procedures at the department of infectious diseases and pulmonary medicine at the Charité -Universitätsmedizin Berlin with informed consents from the patients. The study was approved by the ethical committee of the Charité and all samples were available as residual material without any personal information or clinical data. BALF was centrifuged at 1,200 rpm and supernatants were then collected and stored in aliquots at −80°C until processing for ELISA.