Selected article for: "cell death and virus infection"

Author: Wilkins, Jordan; Zheng, Yi-Min; Yu, Jingyou; Liang, Chen; Liu, Shan-Lu
Title: Nonhuman Primate IFITM Proteins Are Potent Inhibitors of HIV and SIV
  • Document date: 2016_6_3
  • ID: 1m1woscb_13
    Snippet: For HIV-1 long-term replication, 5 X 10 5 SupT1 cells were challenged with appropriate volumes of the wild type NL4.3 viral stock; unbound virus was removed 6 hours after the initiation of infection and cells were incubated with fresh RPMI-1640 medium. Supernatants containing newly produced virions were collected every 2 days until a complete cell death was observed. Supernatants were measured for viral production by performing reverse transcript.....
    Document: For HIV-1 long-term replication, 5 X 10 5 SupT1 cells were challenged with appropriate volumes of the wild type NL4.3 viral stock; unbound virus was removed 6 hours after the initiation of infection and cells were incubated with fresh RPMI-1640 medium. Supernatants containing newly produced virions were collected every 2 days until a complete cell death was observed. Supernatants were measured for viral production by performing reverse transcriptase (RT) assay (see below) and for infectivity by infecting HeLa-TZM-bl cells.

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