Novel Variants of Angiotensin Converting Enzyme-2 of Shorter Molecular Size to Target the Kidney Renin Angiotensin System

Wysocki, Jan; Schulze, Arndt; Batlle, Daniel

The effect of short rACE2 on acute hypertension was examined in a model of angiotensin II-induced hypertension as previously described by us [6, 7, 15] . Mice were pretreated with either vehicle (PBS), rACE2 1-605 or rACE2 1-619, by a single i.p. injection at 1 h before receiving a bolus injection of Ang II (Figure 7 ). Blood pressure in mice was monitored continuously under light anesthesia in a time series at 30 s intervals that started five minutes before Ang II injection (see Methods). The injection of Ang II resulted in a rapid increase in systolic blood pressure (peak of 204 ± 5 and 169 ± 8 mm Hg) from a baseline (124 ± 9 and 114 ± 9 mm Hg) in the control groups that had received PBS as pre-injection vehicle ( Figure 7A,B, respectively) . This increase in SBP was efficiently mitigated to a similar extent in the both rACE2 treatment groups ( Figure 7A,B) .