Selected article for: "complex host background and high throughput"
Author: Gardner, Shea N.; Jaing, Crystal J.; Elsheikh, Maher M.; Peña, José; Hysom, David A.; Borucki, Monica K.
Title: Multiplex Degenerate Primer Design for Targeted Whole Genome Amplification of Many Viral Genomes
  • Document date: 2014_8_3
    • ID: 1e44usl6_30
    Snippet: Software is described to generate tiled, multiplex, and degenerate amplification primers to span entire genomes or regions of many variant sequences. This tool should facilitate the amplification of overlapping products across whole genomes or user-specified regions of target sets with high levels of variation. Applications include target enrichment for viral discovery of new members in a viral family from a complex host background, improving hig.....
    Document: Software is described to generate tiled, multiplex, and degenerate amplification primers to span entire genomes or regions of many variant sequences. This tool should facilitate the amplification of overlapping products across whole genomes or user-specified regions of target sets with high levels of variation. Applications include target enrichment for viral discovery of new members in a viral family from a complex host background, improving high throughput sequencing sensitivity and coverage of a rapidly evolving virus, or enriched coverage of variants in a gene family.

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