NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap(4)A) and Down-Regulates Immune Response and Cancer Promotion Genes

Marriott, Andrew S.; Vasieva, Olga; Fang, Yongxiang; Copeland, Nikki A.; McLennan, Alexander G.; Jones, Nigel J.

MYOC and NEUROG1 was detected so their significance is hard to determine. In the case of MHC-II genes, SMC3, a subunit of the cohesin protein complex, was identified as a possible regulator. Cohesin co-operates with MHC-II specific transcription factors RFX5 and CIITA (up 1.3-fold) to activate MHC-II expression [136] . SMC3 has an ATP-binding domain but whether cohesin binding to MHC-II transcriptional insulators could be promoted by Ap 4 A is unknown. The ubiquitin E3 ligase PDLIM2 (SLIM) is predicted by IPA 1 to both up-regulate and down-regulate different sets of genes (S8 Table) . This prediction appears to be based largely on the results of one previous investigation into PDLIM2-modulated gene expression in DU145 prostate carcinoma cells [137] and the direction of expression change of about half the genes in that study is different from that in ours. Given that the z-scores for the predictions are both below the significance threshold of 2