NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap(4)A) and Down-Regulates Immune Response and Cancer Promotion Genes

Marriott, Andrew S.; Vasieva, Olga; Fang, Yongxiang; Copeland, Nikki A.; McLennan, Alexander G.; Jones, Nigel J.

Ap 4 A has been reported to activate the transcription of subsets of genes controlled by the transcription factors MITF and USF2 [31, 36] . In view of this, and to further explore the phenotype of the NUDT2 knockout cells, we carried out a comparative analysis of the transcriptomes of KBM-7 and NuKO cells by RNA-Seq to identify DEGs. An average of 46.1 million pairs of 100 bp paired-end reads per sample were generated that aligned to the reference human genome. Alignment results are summarized in Table 1 , showing the number and percentage of reads mapped for each sample. Mapping percentages for the six samples were between 80.2 and 81.3%. 31,177 (48.5%) of the 64,253 reference genes had at least one read aligned while 33,076 genes had no read aligned from any of the six samples.