Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches

Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian

The levels of anti-PEDV specific IgG antibody in the serum and SIgA antibody in the feces was determined by ELISA. As shown in the Fig. 6 , the levels of anti-PEDV specific IgG antibody in the serum increased after oral immunization with UV-inactivated PEDV (p < 0.01) as compared to those in the control groups of piglets orally immunized with PBS after the first vaccinations. At 14 and 49 day, anti-PEDV specific IgG antibody in the serum reached a relatively Fig. 3 DCs can stimulate lymphocytes to secrete cytokines. DCs in porcine PPSs from conventional healthy pigs and oral inactivated PEDV for two months co-cultured with allogeneic lymphocytes at a ratio of 1:5 for 24 h respectively, the culture supernatants were collected. IL-2 (a), IL-4 (b), IL-6 (c) and IL-10 (d) release in culture supernatants were measured by ELISA. All of the data are presented as means ± SD of three replicates and are representative of three independent experiments. *P < 0.05; **P < 0.01 higher level (p < 0.01) (Fig. 6a) . Similar variation tendency of SIgA titers was detected in the feces (Fig. 6b) . No significant difference was detected in the negative control group during the experiment.