Selected article for: "growth medium and ml growth"

Author: Marriott, Andrew S.; Vasieva, Olga; Fang, Yongxiang; Copeland, Nikki A.; McLennan, Alexander G.; Jones, Nigel J.
Title: NUDT2 Disruption Elevates Diadenosine Tetraphosphate (Ap(4)A) and Down-Regulates Immune Response and Cancer Promotion Genes
  • Document date: 2016_5_4
  • ID: 0ozzbp85_9
    Snippet: Cells (2 x 10 5 ) were seeded into 25 cm 2 flasks containing 7 mL of growth medium. Chemical agents were added as stated and cells grown for 96 h at 37°C after which cultures were centrifuged at 500 g for 5 min, cells resuspended in fresh medium, and counted using a haemocytometer. Average counts were normalized to the cell count of the untreated culture......
    Document: Cells (2 x 10 5 ) were seeded into 25 cm 2 flasks containing 7 mL of growth medium. Chemical agents were added as stated and cells grown for 96 h at 37°C after which cultures were centrifuged at 500 g for 5 min, cells resuspended in fresh medium, and counted using a haemocytometer. Average counts were normalized to the cell count of the untreated culture.

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