Author: Yuan, Chen; Zhang, En; Huang, Lulu; Wang, Jialu; Yang, Qian
Title: Oral administration of inactivated porcine epidemic diarrhea virus activate DCs in porcine Peyer’s patches Document date: 2018_8_16
ID: 184kmuiy_10
Snippet: After removal of residual mesenteric fat tissue, the ileum was then cut into 1.5 cm pieces. The pieces were incubated in 20 ml of 5 mM EDTA in HBSS for 20 min at 4°C. Then centrifuged and discarded the supernatant. The ileum was cut in 1 cm pieces and placed in digestion solution containing 4% fetal bovine serum, 2 mg/ml each of Collagenase D (Roche) and DNase I (Sigma), and 100 U/ml Dispase (Fisher) at 37°C for 20 min with slow rotation. The s.....
Document: After removal of residual mesenteric fat tissue, the ileum was then cut into 1.5 cm pieces. The pieces were incubated in 20 ml of 5 mM EDTA in HBSS for 20 min at 4°C. Then centrifuged and discarded the supernatant. The ileum was cut in 1 cm pieces and placed in digestion solution containing 4% fetal bovine serum, 2 mg/ml each of Collagenase D (Roche) and DNase I (Sigma), and 100 U/ml Dispase (Fisher) at 37°C for 20 min with slow rotation. The supernatants were obtained by density gradient centrifugation and then sorted DCs marked SWC3a and MHC-IIfrom porcine PPs by fluorescence activated cell sorting (FACS).
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