Author: McCarthy, Mary K.; Reynoso, Glennys V.; Winkler, Emma S.; Mack, Matthias; Diamond, Michael S.; Hickman, Heather D.; Morrison, Thomas E.
Title: MyD88-dependent influx of monocytes and neutrophils impairs lymph node B cell responses to chikungunya virus infection via Irf5, Nos2 and Nox2 Document date: 2020_1_30
ID: 1tut4erh_54
Snippet: The dLN or left foot was dissected and homogenized in TRIzol Reagent (Life Technologies) for RNA analysis with a MagNA Lyser (Roche). RNA was isolated using the PureLink RNA Mini kit (Thermo Fisher) with on-column DNase treatment. Gene expression was quantified by RT-qPCR using available Taqman gene expression assays (Thermo Fisher). Expression of each gene was normalized to 18S and expressed as fold change over mock samples......
Document: The dLN or left foot was dissected and homogenized in TRIzol Reagent (Life Technologies) for RNA analysis with a MagNA Lyser (Roche). RNA was isolated using the PureLink RNA Mini kit (Thermo Fisher) with on-column DNase treatment. Gene expression was quantified by RT-qPCR using available Taqman gene expression assays (Thermo Fisher). Expression of each gene was normalized to 18S and expressed as fold change over mock samples.
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