Author: Sugiarto, Sarah; Spiri, Andrea M.; Riond, Barbara; Novacco, Marilisa; Oestmann, Angelina; de Miranda, Luisa H. Monteiro; Meli, Marina L.; Boretti, Felicitas S.; Hofmann-Lehmann, Regina; Willi, Barbara
Title: Passive immunization does not provide protection against experimental infection with Mycoplasma haemofelis Document date: 2016_8_5
ID: 1uw8xcxw_18
Snippet: RBC OF was determined on day 0 and on days 21, 100 and 179 pi using a published protocol [10] . The reference interval was determined in nine healthy cats during a previous study (50% hemolysis in NaCl 0.50-0.57% w/v) [10] . Direct Coombs tests were performed on day 0 and on days 21 and 100 pi. RBCs from EDTA-anticoagulated blood samples were washed, diluted in NaCl 0.9% w/v solution and incubated for 1 h at 37 °C with feline antiglobulin reagen.....
Document: RBC OF was determined on day 0 and on days 21, 100 and 179 pi using a published protocol [10] . The reference interval was determined in nine healthy cats during a previous study (50% hemolysis in NaCl 0.50-0.57% w/v) [10] . Direct Coombs tests were performed on day 0 and on days 21 and 100 pi. RBCs from EDTA-anticoagulated blood samples were washed, diluted in NaCl 0.9% w/v solution and incubated for 1 h at 37 °C with feline antiglobulin reagent (MP Biomedicals, LLC., Solon, Ohio, USA) in dilutions ranging from 1:2 to 1:10240, as described [10] . Agglutination in dilutions of ≥1:8 was defined as positive.
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