Author: Melnik, Lilia I; Garry, Robert F; Morris, Cindy A
Title: Peptide inhibition of human cytomegalovirus infection Document date: 2011_2_22
ID: 0p6x4lwx_29
Snippet: HFF were seeded at a density of 3.5×10 5 cells in each well of a 24-well plate 24 hours prior to infection. HFF were washed with 1× DPBS and mock-or virus-infected for 90 minutes at RT with the Towne-GFP strain of HCMV (0.5 MOI) preincubated with different concentrations of inhibitory peptides at 37°C for 90 minutes. After infection, virus was removed and Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), p.....
Document: HFF were seeded at a density of 3.5×10 5 cells in each well of a 24-well plate 24 hours prior to infection. HFF were washed with 1× DPBS and mock-or virus-infected for 90 minutes at RT with the Towne-GFP strain of HCMV (0.5 MOI) preincubated with different concentrations of inhibitory peptides at 37°C for 90 minutes. After infection, virus was removed and Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), penicillin G (100 U/mL), streptomycin (100 mg/mL), and GlutaMAX (2 mM) was added to each well and cells were incubated at 37°C for 48 hours. GFP-positive cells were visualized 48 hours post-infection by fluorescence microscopy and then quantified using flow cytometry.
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