Selected article for: "antibody detection and rapid detection"
Author: Colino, Clara I.; Millán, Carmen Gutiérrez; Lanao, José M.
Title: Nanoparticles for Signaling in Biodiagnosis and Treatment of Infectious Diseases
  • Document date: 2018_5_31
    • ID: 1bxwb2hj_52
    Snippet: Immunoassay using fluorescent antibody-nanoparticle conjugates may be applied for the detection of Gram-negative bacteria such as E. coli or P. aeruginosa. Conjugates of functionalized silica nanoparticles with FITC allow for the rapid detection of Escherichia coli O157:H7 by flow cytometry in beef samples. Rabbit anti-E. coli O157:H7 antibody was used to recognize E. coli O157:H7. Protein A, a stable protein extracted from S. aureus, was used fo.....
    Document: Immunoassay using fluorescent antibody-nanoparticle conjugates may be applied for the detection of Gram-negative bacteria such as E. coli or P. aeruginosa. Conjugates of functionalized silica nanoparticles with FITC allow for the rapid detection of Escherichia coli O157:H7 by flow cytometry in beef samples. Rabbit anti-E. coli O157:H7 antibody was used to recognize E. coli O157:H7. Protein A, a stable protein extracted from S. aureus, was used for antibody coupling on silica nanoparticle surfaces. The method is rapid, selective and sensitive for the detection of E. coli [59] .

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