Author: O’Connell, Grant C.; Treadway, Madison B.; Petrone, Ashley B.; Tennant, Connie S.; Lucke-Wold, Noelle; Chantler, Paul D.; Barr, Taura L.
Title: Peripheral blood AKAP7 expression as an early marker for lymphocyte-mediated post-stroke blood brain barrier disruption Document date: 2017_4_26
ID: 1ey6ie95_22
Snippet: For quantitative PCR, target sequences were amplified from 10 ng of cDNA input using custom probe sets designed using the NCBI primer design tool and detected via SYBR green (PowerSYBR, Thermo-Fisher) on the RotorGeneQ (Qiagen). Raw amplification plots were background corrected and CT values were generated via the RotorGeneQ software package. All reactions were performed in triplicate. Transcripts of B2M, PPIB, and ACTB were amplified as low vari.....
Document: For quantitative PCR, target sequences were amplified from 10 ng of cDNA input using custom probe sets designed using the NCBI primer design tool and detected via SYBR green (PowerSYBR, Thermo-Fisher) on the RotorGeneQ (Qiagen). Raw amplification plots were background corrected and CT values were generated via the RotorGeneQ software package. All reactions were performed in triplicate. Transcripts of B2M, PPIB, and ACTB were amplified as low variability references and normalization was performed using the NORMAgene data-driven normalization algorithm 43 . Expression values are presented as fold difference relative to control in the case of groupwise comparisions, or as fold value relative to lowest expression for correlational analysis.
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