Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids

Li, Zhao; Liu, Yong; Wei, Qingquan; Liu, Yuanjie; Liu, Wenwen; Zhang, Xuelian; Yu, Yude

Recently, a number of reports have proposed RPA-based strategies for pathogen detection. The detection formats include fluorescence detection in real time or endpoint detection via a lateral flow strip [52] [53] [54] [55] . Reverse transcription RPA can be also used to detect RNA targets from foot-and-mouth disease virus or Middle East respiratory syndrome coronavirus [56, 57] . Additionally, RPA is highly sensitive for the detection of HIV proviral DNA and Mycobacterium tuberculosis DNA [52, 55, 58] . Instrument-free and electricity-free RPA can be performed using body heat or heating achieved using sodium acetate trihydrate for nucleic acid diagnostic tests in low-resource settings that lack expensive thermal cycling PCR equipment or even electric power [59, 60] .