Author: Li, Zhao; Liu, Yong; Wei, Qingquan; Liu, Yuanjie; Liu, Wenwen; Zhang, Xuelian; Yu, Yude
Title: Picoliter Well Array Chip-Based Digital Recombinase Polymerase Amplification for Absolute Quantification of Nucleic Acids Document date: 2016_4_13
ID: 0tmd9knh_9
Snippet: The L. monocytogenes gDNA stock solution was prepared by rehydrating the dried DNA powder with DNase/RNase-free water. The final concentration was 40 pg μL −1 , which was verified using a NanoDrop spectrophotometer (Thermo Fisher Scientific). One picogram of L. monocytogenes gDNA contained approximately 315 copies. The volume of reaction solution in each microwell was 314 pL, and for a gDNA sample concentration of 40 pg μL -1 , approximately .....
Document: The L. monocytogenes gDNA stock solution was prepared by rehydrating the dried DNA powder with DNase/RNase-free water. The final concentration was 40 pg μL −1 , which was verified using a NanoDrop spectrophotometer (Thermo Fisher Scientific). One picogram of L. monocytogenes gDNA contained approximately 315 copies. The volume of reaction solution in each microwell was 314 pL, and for a gDNA sample concentration of 40 pg μL -1 , approximately 9 × 10 -1 copies per well was expected. The detailed description of the calculating method is shown in S1 Fig.
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