Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2 Document date: 2019_1_17
ID: 1c5ug64m_15
Snippet: The specificity of the PCR-LFIA was established by testing three known positive strains (CPV-2-N1, CPV-2-N2, and CPV-2-N3) and several non-CPV strains (PRV-R1, CDV-NJ2, CCoV-C5, CPIV-J2) maintained in our laboratory. Previous study has verified the specificity of the PCR method based on its specific primers, the focus here is on verifying the specificity of PCR-LFIA [32] . Viral RNA was translated to cDNA by PrimeScriptâ„¢ RT Master Mix (TaKaRa, .....
Document: The specificity of the PCR-LFIA was established by testing three known positive strains (CPV-2-N1, CPV-2-N2, and CPV-2-N3) and several non-CPV strains (PRV-R1, CDV-NJ2, CCoV-C5, CPIV-J2) maintained in our laboratory. Previous study has verified the specificity of the PCR method based on its specific primers, the focus here is on verifying the specificity of PCR-LFIA [32] . Viral RNA was translated to cDNA by PrimeScriptâ„¢ RT Master Mix (TaKaRa, Dalian, China) before PCR. Each sample test was repeated three times.
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