Selected article for: "frame ORF reading and reading frame"
Author: de Miranda, Joachim R.; Hedman, Harald; Onorati, Piero; Stephan, Jörg; Karlberg, Olof; Bylund, Helena; Terenius, Olle
Title: Characterization of a Novel RNA Virus Discovered in the Autumnal Moth Epirrita autumnata in Sweden
  • Document date: 2017_8_8
    • ID: 1baso3q2_10
    Snippet: The Abiskovirus genomic (positive) and replicative (negative) RNA strands were quantified separately using four pairs of strand-specific assays; two located in open reading frame 1 (ORF-1) and two located in ORF-2/3. Strand-specific cDNA was generated in 10 μL reactions containing 1 μL RNA plus 2 μM tagged cDNA primer (Supplementary Materials Table S1) heated to 70 °C for 5 min and cooled on ice; 20u MuMLV reverse transcriptase (Thermo Fisher.....
    Document: The Abiskovirus genomic (positive) and replicative (negative) RNA strands were quantified separately using four pairs of strand-specific assays; two located in open reading frame 1 (ORF-1) and two located in ORF-2/3. Strand-specific cDNA was generated in 10 μL reactions containing 1 μL RNA plus 2 μM tagged cDNA primer (Supplementary Materials Table S1) heated to 70 °C for 5 min and cooled on ice; 20u MuMLV reverse transcriptase (Thermo Fisher K1612, Waltham, MA, USA), 10u RiboLock RNAse inhibitor (Thermo Fischer EO03819), 1 mM dNTP plus the MuMLV 5x reaction buffer. For each sample, a no-template, a no-primer, and a no-reverse transcriptase reaction were included in its suite of cDNA reactions, as essential cDNA negative controls [11, 13] . Also included

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