Selected article for: "agarose gel electrophoresis and characteristic peak"
Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2
  • Document date: 2019_1_17
    • ID: 1c5ug64m_23
    Snippet: In order to evaluate the specificity of PCR-LFIA, potential cross-reactions were performed using DNA/RNA three positive strains (CPV-2-N1, CPV-2-N2, and CPV-2-N3) and several non-CPV strains (PRV-R1, CDV-NJ2, CCoV-C5, CPIV-J2) were tested in this study. PCR products were analyzed by 1.5% agarose gel electrophoresis and LFIA. As shown in Fig. 4a , cross-amplification tests using templates from PRV, CDV, CCoV and CPIV showed that no amplicons were .....
    Document: In order to evaluate the specificity of PCR-LFIA, potential cross-reactions were performed using DNA/RNA three positive strains (CPV-2-N1, CPV-2-N2, and CPV-2-N3) and several non-CPV strains (PRV-R1, CDV-NJ2, CCoV-C5, CPIV-J2) were tested in this study. PCR products were analyzed by 1.5% agarose gel electrophoresis and LFIA. As shown in Fig. 4a , cross-amplification tests using templates from PRV, CDV, CCoV and CPIV showed that no amplicons were detected, whereas the reaction using the CPV-2 template gave a positive result. The quantitative results were also obtained by Nanoeasy 1700 Fig. 5a . c Fluorescence signal values as a function of the copies of standard DNA detected. Each value was derived from three independent detections, and the error bars mean standard deviations (Table 2) , and the specific characteristic peak appeared only for CPV-2 detection (Fig. 4b) . These results indicated that the PCR-LFIA developed in this study was specific for CPV-2.

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