Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu

Parvovirus infections in dogs have become a global problem. The clinical signs resemble other enteric diseases and hence rapid and early diagnosis of the condition has become ever more urgent. Traditional methods such as virus isolation and electron microscopy are time-consuming, less sensitive, and expensive [35, 36] . Serological tests could detect the antibody, but fail to detect the acute infection. The commercial SNAP test is commonly used for rapid detection of CPV-2 on site, but it was less sensitive than PCR-based assays because it does not amplify the detection target during the test [7] [8] [9] 37] . Haemagglutination and immunochromatographic tests are widely used and simple, but they are less sensitive and always require fresh samples [8, 36] . Hence, these tests are now replaced by molecular methods like PCR which has high specificity and sensitivity than the traditional methods [38, 39] . However, the necessity of expensive equipment and reagents (real-time PCR) or cumbersome electrophoresis equipment and condition (PCR-GE) restricts its wide application [40] .