Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu

Specific and sensitive laboratory diagnostic methods available for CPV detection have been developed over the years. Routinely, immunological-based methods such as enzyme-linked immunosorbent assay (ELISA), immunochromatographic (IC) and haemagglutination (HA) have been used to screen faeces from diarrhoeic dogs, but these methods are affected by relatively low sensitivity [9] [10] [11] . Virus isolation (VI) is more sensitive and accurate, while it is too time-consuming and laborious for pathogen detection [12] . With advances in molecular techniques, conventional and real-time PCR assays were gradually developed for CPV-2 detection with higher sensitivity and specificity [13] [14] [15] . And further study showed that among above methods for detection of CPV-2, the best correlation was found between conventional and real-time PCR [8] . However, real-time PCR has not spread to the primary detection method due to its high equipment and reagent cost. Alternatively, conventional PCR assay based on agarose gel electrophoresis (PCR-GE), followed by nucleic acid dyes (such as ethidium bromide) staining, which poses a great potential threat to the health of the experimenter. All of these factors may have limited suitability for wide application.