Selected article for: "luciferase activity and reporter plasmid"
Author: Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing
Title: Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway
  • Document date: 2015_8_18
    • ID: 0qt7eth0_11
    Snippet: It is possible that PEDV blocks poly (I:C)-mediated IFNβ production by suppression of the individual molecules upstream of TBK1/IKKε in RIG-I signaling pathway. To explore this possibility, mock-and PEDV-infected IECs were cotransfected with IPS-1 expression plasmid and IFN-β promoter luciferase reporter plasmid, respectively. As shown in Fig. 4a , overexpression of IPS-1 in IECs could enhance IFN-β luciferase activity in mock-infected cells,.....
    Document: It is possible that PEDV blocks poly (I:C)-mediated IFNβ production by suppression of the individual molecules upstream of TBK1/IKKε in RIG-I signaling pathway. To explore this possibility, mock-and PEDV-infected IECs were cotransfected with IPS-1 expression plasmid and IFN-β promoter luciferase reporter plasmid, respectively. As shown in Fig. 4a , overexpression of IPS-1 in IECs could enhance IFN-β luciferase activity in mock-infected cells, but it was completely inhibited in PEDV-infected cells. For the poly (I:C) transfection experiments, there appeared significant restriction of IFN-β luciferase expression in PEDV-infected cells compared with that of mock-infected cells. These date indicated that PEDV interacted with IPS-1 to block poly (I:C)-mediated IFN-β transcription.

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