Selected article for: "dual luciferase and expression vector"

Author: Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing
Title: Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway
  • Document date: 2015_8_18
  • ID: 0qt7eth0_14
    Snippet: In summary, the findings of the present study suggested that PEDV-infection in IECs inhibits dsRNA-induced IFN-β induction by interfering with IRF-3 activity associated with RIG-I-mediated signaling pathway. The target interaction molecules of PEDV intervention of dsRNAinduced IFN-β production primarily was IPS-1. However, as a limitation to this study, host cells may inhibit or activate multiple signaling pathways simultaneously in response to.....
    Document: In summary, the findings of the present study suggested that PEDV-infection in IECs inhibits dsRNA-induced IFN-β induction by interfering with IRF-3 activity associated with RIG-I-mediated signaling pathway. The target interaction molecules of PEDV intervention of dsRNAinduced IFN-β production primarily was IPS-1. However, as a limitation to this study, host cells may inhibit or activate multiple signaling pathways simultaneously in response to exogenous stimulus, and some other transcription factors may have been blocked or activated in this process. Here, we only addressed the mechanisms of PEDV-induced inhibition of IFN-β production in relation to the molecules of RIG-I signaling pathways in vitro. Further studies are needed. Overall, elucidation of the Cells were harvested and subjected to a dual-luciferase assay. Data were analyzed and the ratio of firefly luciferase expression to Renilla luciferase activity was shown. All data are expressed as means ± SD of 3 independent experiments. **p < 0.01 compared with PEDV-infected, expression plasmids or vector-transfected control influence of PEDV evasion of the host innate immune response will aid in the development of antiviral agents to prevent the spread of PEDV during the early infection phase.

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