Selected article for: "actin antibody and ma beverly signaling technology"

Author: Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing
Title: Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway
  • Document date: 2015_8_18
  • ID: 0qt7eth0_17
    Snippet: Poly (I:C) was purchased as a sodium salt (Sigma-Alorch, Saint Louis, MO, USA) and dissolved in water to obtain a stock solution of 10 mg/mL. The Dual-Luciferase® Reporter Assay System was purchased from Promega Corporation (Madison, WI, USA) and monoclonal anti-β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-IRF-3, anti-p65 anti-p-IRF-3 and anti-p-p65 rabbit polyclonal antibodies and secondary horseradish peroxidas.....
    Document: Poly (I:C) was purchased as a sodium salt (Sigma-Alorch, Saint Louis, MO, USA) and dissolved in water to obtain a stock solution of 10 mg/mL. The Dual-Luciferase® Reporter Assay System was purchased from Promega Corporation (Madison, WI, USA) and monoclonal anti-β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-IRF-3, anti-p65 anti-p-IRF-3 and anti-p-p65 rabbit polyclonal antibodies and secondary horseradish peroxidase (HRP)-conjugated anti-rabbit IgG were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Rhodamine isothiocyanate (TRITC)-labeled goat anti-rabbit IgG were purchased from the Zhongshan Company (Beijing, China). Anti-PEDV rabbit polyclonal antibodies and anti-PEDV N protein monoclonal antibody (McAb) were prepared in our laboratory, which could specifically react with PEDV.

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