Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway

Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing

Poly (I:C) was purchased as a sodium salt (Sigma-Alorch, Saint Louis, MO, USA) and dissolved in water to obtain a stock solution of 10 mg/mL. The Dual-Luciferase® Reporter Assay System was purchased from Promega Corporation (Madison, WI, USA) and monoclonal anti-β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-IRF-3, anti-p65 anti-p-IRF-3 and anti-p-p65 rabbit polyclonal antibodies and secondary horseradish peroxidase (HRP)-conjugated anti-rabbit IgG were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Rhodamine isothiocyanate (TRITC)-labeled goat anti-rabbit IgG were purchased from the Zhongshan Company (Beijing, China). Anti-PEDV rabbit polyclonal antibodies and anti-PEDV N protein monoclonal antibody (McAb) were prepared in our laboratory, which could specifically react with PEDV.