Selected article for: "activity essential and luciferase reporter"
Author: Cao, Liyan; Ge, Xuying; Gao, Yu; Herrler, Georg; Ren, Yudong; Ren, Xiaofeng; Li, Guangxing
Title: Porcine epidemic diarrhea virus inhibits dsRNA-induced interferon-ß production in porcine intestinal epithelial cells by blockade of the RIG-I-mediated pathway
  • Document date: 2015_8_18
    • ID: 0qt7eth0_9
    Snippet: PEDV failed to block TBK1/IKKε activity TBK1 and IKKε are essential kinases for the IRF-3 activation [29] . In order to ascertain whether PEDV inhibited poly (I:C)-induced IRF-3 activation by impeding TBK1/IKKε kinase activity, we cotransfected plasmids expressing TBK1/IKKε kinase and a plasmid encoding the IFN-β promoter of the luciferase reporter into infected or mock-infected IECs and retransfected the cells with or without poly (I:C) at .....
    Document: PEDV failed to block TBK1/IKKε activity TBK1 and IKKε are essential kinases for the IRF-3 activation [29] . In order to ascertain whether PEDV inhibited poly (I:C)-induced IRF-3 activation by impeding TBK1/IKKε kinase activity, we cotransfected plasmids expressing TBK1/IKKε kinase and a plasmid encoding the IFN-β promoter of the luciferase reporter into infected or mock-infected IECs and retransfected the cells with or without poly (I:C) at 36 h.p.i. As show in Fig. 3 , TBK1/IKKε overexpression increased IFN-β promoter activity in both infected and mock-infected IECs, and obviously upregulation was detected in IECs transfected with poly (I:C), suggesting that PEDV failed to block TBK1/IKKε activity. However, poly (I:C)-induced IFN-β promoter activity in IECs overexpression of TBK1/ IKKε plasmids was significantly inhibited by PEDV. It showed that PEDV interrupting dsRNA-induced IFN-β production should localize upstream from TBK1/IKKε.

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