Author: Wilkins, Jordan; Zheng, Yi-Min; Yu, Jingyou; Liang, Chen; Liu, Shan-Lu
Title: Nonhuman Primate IFITM Proteins Are Potent Inhibitors of HIV and SIV Document date: 2016_6_3
ID: 1m1woscb_17
Snippet: SupT1 cells were seeded at 4 X10 5 cells/ml in 12-well dishes. Infectious virus was added in the presence of 4 μg/ml polybrene with spinoculation at 1,850 X g for 1 hour at 4˚C and then incubated at 37˚C for 6 hours. SupT1 cells were then refed and incubated for an additional 48 hours. Viral supernatants were used to infect HeLa-TZM-bl cells. Forty-eight hours post-infection, HeLa-TZM-bl cells were harvested in lysis buffer (50 mM Tris pH 7.4,.....
Document: SupT1 cells were seeded at 4 X10 5 cells/ml in 12-well dishes. Infectious virus was added in the presence of 4 μg/ml polybrene with spinoculation at 1,850 X g for 1 hour at 4˚C and then incubated at 37˚C for 6 hours. SupT1 cells were then refed and incubated for an additional 48 hours. Viral supernatants were used to infect HeLa-TZM-bl cells. Forty-eight hours post-infection, HeLa-TZM-bl cells were harvested in lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100). HeLa-TZM-bl lysates were mixed at a 1:1 ratio with assay buffer (200 mM Tris pH 7.8, 250 mM MgCl2, 7.5 mM ATP, 25 mM CoA, 0.2 mg/ml D-Luciferin) and measured for luciferase activity using a Perkin Elmer plate reader.
Search related documents:
Co phrase search for related documents- HeLa TZM bl and TZM bl: 1, 2, 3, 4
- HeLa TZM bl cell and SupT1 cell: 1
- HeLa TZM bl cell and TZM bl: 1
- hour post infection and post infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- infectious virus and luciferase activity: 1, 2, 3, 4, 5, 6, 7, 8
- infectious virus and lysis buffer: 1, 2, 3, 4
- infectious virus and Perkin Elmer plate: 1
- infectious virus and Perkin Elmer plate reader: 1
- infectious virus and Perkin Elmer plate reader luciferase activity: 1
- infectious virus and post infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- infectious virus and viral supernatant: 1, 2, 3, 4, 5, 6
- luciferase activity and lysis buffer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- luciferase activity and Perkin Elmer plate: 1
- luciferase activity and Perkin Elmer plate reader: 1
- luciferase activity and Perkin Elmer plate reader luciferase activity: 1
- luciferase activity and post infection: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- luciferase activity and TZM bl: 1, 2, 3, 4, 5, 6, 7
- lysis buffer and post infection: 1, 2, 3, 4, 5
- lysis buffer and TZM bl: 1
Co phrase search for related documents, hyperlinks ordered by date