Selected article for: "amino acid and nucleotide level"

Author: Wilkins, Jordan; Zheng, Yi-Min; Yu, Jingyou; Liang, Chen; Liu, Shan-Lu
Title: Nonhuman Primate IFITM Proteins Are Potent Inhibitors of HIV and SIV
  • Document date: 2016_6_3
  • ID: 1m1woscb_25
    Snippet: For this study, 14 new nonhuman primate IFITM sequences were used (see under Cloning and DNA Sequencing), among which 13 are unique sequences (6 IFITM1 and 7 IFITM3) at the nucleotide level and 11 are unique sequences (4 IFITM1 and 7 IFITM3) at the amino acid level. A total of 18 nucleotide sequences were used for IFITM analysis within Datamonkey online interface (http://www.datamonkey.org/dataupload.php) and MEGA6 software [44] . Sequences were .....
    Document: For this study, 14 new nonhuman primate IFITM sequences were used (see under Cloning and DNA Sequencing), among which 13 are unique sequences (6 IFITM1 and 7 IFITM3) at the nucleotide level and 11 are unique sequences (4 IFITM1 and 7 IFITM3) at the amino acid level. A total of 18 nucleotide sequences were used for IFITM analysis within Datamonkey online interface (http://www.datamonkey.org/dataupload.php) and MEGA6 software [44] . Sequences were aligned using ClustalW and a phylogeny of IFITM was constructed by the Neighbor-joining method [45, 46] . Evolutionary distances were estimated using the Jukes Cantor method [47] . Confidence levels were determined using the bootstrap method with 1000 replicates and selection was determined using the Nei-Gojobori method [48] .

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