Author: Pan, Yen-Yu; Wang, Shiu-Mei; Huang, Kuo-Jung; Chiang, Chien-Cheng; Wang, Chin-Tien
Title: Placement of Leucine Zipper Motifs at the Carboxyl Terminus of HIV-1 Protease Significantly Reduces Virion Production Document date: 2012_3_1
ID: 09locmnw_20
Snippet: In an assembly-competent Gag/PR context, the wt LZ insertion resulted in markedly reduced, PR activity-dependent virus production (Fig. 2) . Cellular Pr55 gag was barely detected in PRWWz at 12 and 24 h post-transfection, while most Pr55 gag remained unprocessed in wt transfectants 12 h post-transfection (data not shown). Combined, these results suggest that LZenhanced Gag cleavage is associated with premature Gag processing due to premature PR a.....
Document: In an assembly-competent Gag/PR context, the wt LZ insertion resulted in markedly reduced, PR activity-dependent virus production (Fig. 2) . Cellular Pr55 gag was barely detected in PRWWz at 12 and 24 h post-transfection, while most Pr55 gag remained unprocessed in wt transfectants 12 h post-transfection (data not shown). Combined, these results suggest that LZenhanced Gag cleavage is associated with premature Gag processing due to premature PR activation. We suggest that when fused at the PR C-terminus, the LZ domain facilitates PR-PR interaction via enhanced interactions among MA-CA-NC-PR-LZ chimeras. Gag is subsequently cleaved by PR in trans, either by PR embedded in the chimera, or by a mature and fully processed PR dimer.
Search related documents:
Co phrase search for related documents- Gag cleavage and pr activation: 1, 2, 3, 4, 5, 6, 7, 8
- Gag PR context and pr activation: 1, 2, 3, 4
- Gag processing and LZ domain: 1, 2, 3
- Gag processing and pr activation: 1, 2, 3, 4, 5, 6
Co phrase search for related documents, hyperlinks ordered by date