Author: Zhuang, Linlin; Ji, Yongxin; Tian, Peilong; Wang, Kaixuan; Kou, Chengkun; Gu, Ning; Zhang, Yu
Title: Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2 Document date: 2019_1_17
ID: 1c5ug64m_13
Snippet: The final reaction volume for LFIA was optimized by adding the running buffer of 40 μL, 60 μL, 80 μL, 100 μL and 120 μL to the sample tube containing about 20 μL eluant, followed by mixing for 10 s. Then, the mixed solution was added to the sampling window of the fluorescent lateral flow strip. The reaction time was optimized by incubating the mixture for 60 s, 90 s, 120 s, 150 s, 180 s at room temperature. Finally, the images of test (T) a.....
Document: The final reaction volume for LFIA was optimized by adding the running buffer of 40 μL, 60 μL, 80 μL, 100 μL and 120 μL to the sample tube containing about 20 μL eluant, followed by mixing for 10 s. Then, the mixed solution was added to the sampling window of the fluorescent lateral flow strip. The reaction time was optimized by incubating the mixture for 60 s, 90 s, 120 s, 150 s, 180 s at room temperature. Finally, the images of test (T) and control (C) lines were recorded by fluorescence imager (Nanoeast, Nanjing, China) and the related fluorescent signal values were read by LFIA reader Nanoeasy 1700 (Nanoeast, Nanjing, China).
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